10/31/2023 0 Comments Purify app facebook![]() ![]() In each coupling cycle, a low percentage of the oligonucleotide chains do not extend, resulting in a mixture of full-length (n) and truncated (n-1, n-2, etc.) sequences (‘shortmers’). During DNA manufacturing, each nucleotide is coupled sequentially to the growing chain via phosphoramidite chemistry.
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